Probe-based mass spectrometry approaches for single-cell and single-organelle measurements

4Citations
Citations of this article
6Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Exploring the chemical content of individual cells not only reveals underlying cell-to-cell chemical heterogeneity but is also a key component in understanding how cells combine to form emergent properties of cellular networks and tissues. Recent technological advances in many analytical techniques including mass spectrometry (MS) have improved instrumental limits of detection and laser/ion probe dimensions, allowing the analysis of micron and submicron sized areas. In the case of MS, these improvements combined with MS's broad analyte detection capabilities have enabled the rise of single-cell and single-organelle chemical characterization. As the chemical coverage and throughput of single-cell measurements increase, more advanced statistical and data analysis methods have aided in data visualization and interpretation. This review focuses on secondary ion MS and matrix-assisted laser desorption/ionization MS approaches for single-cell and single-organelle characterization, which is followed by advances in mass spectral data visualization and analysis.

References Powered by Scopus

Random forests

94865Citations
N/AReaders
Get full text

Fast unfolding of communities in large networks

15007Citations
N/AReaders
Get full text

Finding community structure in networks using the eigenvectors of matrices

3800Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Advances in multimodal mass spectrometry for single-cell analysis and imaging enhancement

4Citations
N/AReaders
Get full text

Advances in cellular and tissue-based imaging techniques for sarcoid granulomas

1Citations
N/AReaders
Get full text

Enhancing lipid production in plant cells through automated high-throughput genome engineering and phenotyping

0Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Castro, D. C., Chan-Andersen, P., Romanova, E. V., & Sweedler, J. V. (2024, July 1). Probe-based mass spectrometry approaches for single-cell and single-organelle measurements. Mass Spectrometry Reviews. John Wiley and Sons Inc. https://doi.org/10.1002/mas.21841

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 2

50%

Professor / Associate Prof. 1

25%

Researcher 1

25%

Readers' Discipline

Tooltip

Physics and Astronomy 2

50%

Biochemistry, Genetics and Molecular Bi... 1

25%

Immunology and Microbiology 1

25%

Save time finding and organizing research with Mendeley

Sign up for free