Reverse genetics

Abstract

Reverse genetics is a technology by which function of a previously unknown gene could be identified by means of mutational studies. It is opposite to classical genetics in which a mutant phenotype will be analyzed for its sequence changes. It is a sophisticated tool for biologist to investigate the function of a gene of interest from various organisms. Reverse genetics is one of the powerful tools in molecular biology and its applications to modern science are endless. The major organisms utilized for the reverse genetics are viruses, bacteria, yeast, worms, mice, and plants.