Design of base-discriminating fluorescent (BDF) nucleobase for SNP typing.

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Abstract

We have devised novel pyrene-labeled BDF nucleosides, (Py)U, (Py)C, (Py)deazaA, and (Py)A. BDF probes containing these fluorescent nucleosides selectively emit fluorescence only when the base opposite the BDF base is a target base. Oligonucleotides containing these BDF nucleosides act as effective reporter probes for homogeneous SNP typing of DNA samples.

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Saito, Y., Kanatani, K., Ochi, Y., Okamoto, A., & Saito, I. (2004). Design of base-discriminating fluorescent (BDF) nucleobase for SNP typing. Nucleic Acids Symposium Series (2004), (48), 243–244. https://doi.org/10.1093/nass/48.1.243

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