Protective effect of sodium bicarbonate on radiological contrast medium-induced nephropathy in rats

Abstract

Radiological iodinated contrasts (IC) agents cause acute kidney injury (AKI). To evaluate the renoprotective eff ect of sodium bicarbonate (Bic) on renal function (creatinine clearance [Clcr], Jaff é, and Clcr mL•min-1×100 g-1) and the oxidative profi le (peroxide excre- tion, urinary peroxides, urinary malondialdehyde, FOX-2 expression, and thiobarbituric acid reactive substance [TBARS; nmol/mg Cr]) in rats treated with an IC agent. Adult male Wistar rats weighing 250-300 g were treated once daily for 5 days with one of the following treatments: saline (0.9%, 3 mL•kg-1?day-1intraperitoneally [i.p.]), IC agent (sodium and meglumine ioxitalamate, 3 mL/kg, i.p.), Bic + Saline (3-mL/kg Bic, i.p., 1 h before and atier saline treatment), and Bic + IC (3-mL/kg Bic, i.p., 1 h before and atier the IC treatment). The IC agent induced AKI, and the antioxidant renoprotective effect of Bic was confirmed (Clcr/TBARS/urinary peroxide: saline group, 0.59 ± 0.03/0.11 ± 0.02/1.29 ± 0.24; Bic + Saline group, 0.58 ± 0.03/0.13 ± 0.02/1.32 ± 0.64; IC group, 0.22 ± 0.02/0.19 ± 0.02/4.77 ± 0.24; Bic + CI group, 0.51 ± 0.04/0.13 ± 0.3/1.80 ± 0.04; p<0.05). The protective eff ect of Bic in the ICinduced AKI was confi rmed; hence, Bic administra tion may be considered as a therapeutic option for patients undergoing IC-enhanced radiography.