Stable isotope labeled cytochrome c 3 from desulfovibrio vulgaris on a defined medium as sole nitrogen source

Abstract

The conditions of cultivation and the composition of medium for Desulfovibrio vulgaris Miyazaki F (DvMF) were examined to obtain cytochrome c3 labeled with a stable isotope. The growth of DvMF was steady and reproducible under purging with N2 and under pH control. DvMF was able to grow on a defined medium without natural products. The composition of medium containing a small amount of NH4Cl as sole nitrogen source was established. Then, [U-15N]cytochrome c3 was obtained during the culture of DvMF in a defined medium with 15NH4Cl; it was confirmed by 1H−15N HMQC. This is the first report of [U-15N]cytochrome c3. © 1996, Taylor & Francis Group, LLC. All rights reserved.