Solar ultraviolet radiation (UVR), through the formation of DNA photolesions, is the primary cause of most skin cancers. A better understanding of the mechanisms of UVR-induced DNA damage may help prevent skin cancer and this may be achieved using methods to quantify DNA damage. The immuno-slot blot (ISB) method is routinely used for detection and quantification of any heat-and alkali-stable DNA adducts for which a sufficiently specific monoclonal antibody is available. The main steps in ISB are fragmentation and denaturation of the DNA, immobilization of DNA to a nitrocellulose filter, incubation with primary antibody against a specific DNA adduct, incubation with an enzyme-linked secondary antibody and finally chemiluminescence detection and quantification of the DNA adducts. © 2012 Springer Science+Business Media New York.
CITATION STYLE
Karbaschi, M., Brady, N. J., Evans, M. D., & Cooke, M. S. (2012). Immuno-slot blot assay for detection of UVR-mediated DNA damage. Methods in Molecular Biology, 920, 163–175. https://doi.org/10.1007/978-1-61779-998-3_12
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