With the identification of Lgr5 as a definitive marker for intestinal stem cells, we used the highly novel, recently described, Lgr5-EGFP-IRES-cre ER T2 knock in mouse model. Mice were injected with azoxymethane (AOM, a colon carcinogen) or saline (control) and fed a chemo-protective diet containing n-3 fatty acids and fermentable fiber (n-3 PUFA+pectin) or a control diet (n-6 PUFA + cellulose). Single cells were isolated from colonic mucosa crypts and three discrete populations of cells were collected via fluorescence activated cell sorting (FACS): Lgr5 high (stem cells), Lgr5 low (daughter cells) and Lgr5 negative (differentiated cells). microRNA profiling and RNA sequencing were performed from the same sample and analyzed. These data refer to 'Comparative effects of diet and carcinogen on microRNA expression in the stem cell niche of the mouse colonic crypt' (Shah et al., 2016) .
Shah, M. S., Kim, E., Davidson, L. A., Knight, J. M., Zoh, R. S., Goldsby, J. S., … Chapkin, R. S. (2016). Data describing the effects of dietary bioactive agents on colonic stem cell microRNA and mRNA expression. Data in Brief, 6, 398–404. https://doi.org/10.1016/j.dib.2015.12.026