Functional glycomics : methods and protocols

Abstract

The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships. In Functional Glycomics: Methods and Protocols, a panel of world-renowned experts provide new developments and emerging glycomics techniques in the form of detailed protocols exploring the fundamental challenges and most cutting-edge novel applications. Written in the highly successful Methods in Molecular Biology series format, chapters in this volume present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Functional Glycomics: Methods and Protocols serves as an ideal reference for scientists working in biochemistry, molecular biology, cell biology, immunology, microbiology, and virology and a guide to appropriate techniques and the design of achievable research plans in this vital field. Functional glycomics and glycobiology: An overview / J. Li and J.C. Richards -- Historical overview of glycoanalysis / A.M. Bielik and J. Zaia -- Quantitative glycomics / R. Orlando -- Human gangliosides and bacterial lipo-oligosaccharides in the development of autoimmune neuropathies / N. Yuki -- Biotinylated multivalent glycoconjugates for surface coating / A.A. Chinarev, O.E. Galanina and N.V. Bovin -- Profiling LPS glycoforms of non-typeable Haemophilus influenzae by multiple-stage tandem mass spectrometry / E.K.H. Schweda and J.C. Richards -- In vitro reconstitution of Escherichia coli O86 O antigen repeating unit / W. Han [and others] -- Glycoprotein characterization / S.M. Twine, L. Tessier and J.F. Kelly -- N-linked glycoprotein analysis using dual-extraction ultrahigh-performance liquid chromatography and electrospray tandem mass spectrometry / S.O. Siu [and others] -- Microarray-based study of carbohydrate-protein binding / Z. Wang and J. Gao -- The application of NMR spectroscopy to functional glycomics / J.-R. Brisson [and others] -- Metabolomics in glycomics / E.C. Soo and J.P.M. Hui -- Characterization of lipid-linked oligosaccharides by mass spectrometry / C.W. Reid, J. Stupak and C.M. Szymanski -- A glycomics approach to the discovery of potential cancer biomarkers / H.J. An and C.B. Lebrilla -- Extraction of chondroitin/dermatan sulfate glycosaminoglycans from connective tissue for mass spectrometric analysis / A.M. Bielik and J. Zaia -- N-linked protein glycosylation in a bacterial system / H. Nothaft [and others] -- Characterization of polysaccharides using mass spectrometry for bacterial serotyping / E. Altman and J. Li -- Is permethylation strategy always applicable to protein N-glycosylation study?: A case study on the O-acetylation of sialic acid in fish serum glycans / X. Liu and L. Afonso -- Bioinformatics in glycomics: Glycan characterization with mass spectrometric data using SimGlycan / A. Apte and N.S. Meitei.