Pathways of organic carbon utilization in small lakes: Results from a whole-lake 13C addition and coupled model

Abstract

In many small aquatic ecosystems, watershed loading of organic C exceeds autochthonous primary production. Although this allochthonous organic C has long been thought of as refractory, multiple lines of evidence indicate that substantial portions are respired in the receiving aquatic ecosystem. To what extent does this terrestrial C support secondary production of invertebrates and fish? Do current models adequately trace the pathways of allochthonous and autochthonous C through the food web? We evaluated the roles of allochthonous and autochthonous organic C by manipulating 13C content of dissolved inorganic C in a small, softwater, humic lake, thereby labeling autochthonous primary production for about 20 d. To ensure rapid and sufficient uptake of inorganic 13C, we enriched the lake with modest amounts of N and P. We constructed a carbon flow model based on the ambient and manipulated levels of 13C in C compartments in the lake, along with information on key rate processes. Despite the short nature of this experiment, several results emerged. (1) Fractionation of photosynthetically assimilated 13C-CO2 by phytoplankton (ε) is lower (∼6‰) than physiologic models would estimate (∼20‰). (2) Bacteria respire, but do not assimilate, a large amount of terrestrially derived dissolved organic C (DOC) and pass little of this C to higher trophic levels. (3) The oxidation of terrestrial DOC is the major source of dissolved inorganic C in the lake. (4) Zooplankton production, a major food of young-of-year fishes, is predominantly derived from current autochthonous carbon sources under the conditions of this experiment.