Identification of DNA corpse in ordo diptera familia calliphoridae larvae in CSF1PO, TH01, and TPOX locus using polymerase chain reaction (PCR) methods

Abstract

Introduction: Biomolecular techniques using DNA are known to have high accuracy in the identification of decaying bodies. In the process of identification, larvae found in corpses that have undergone decay can be used as alternative biological evidence to estimate the time of death. Aim: To identify the transfer of body DNA to the larvae of the fly ordo Diptera family Calliphoridae and the process of DNA transfer of decomposed bodies. Method: blood from the postmortem corpse was taken as a control, then soft tissue was taken as a decomposition product for the breeding of Dipdo family Calliphoridae larvae. The protocol used is DNA profiling using the Short Tandem Repeat (STR) locus such as CSF1PO, TH01, and TPOX. The results of DNA extraction from the postmortem body and DNA extraction results obtained from the skin of the larvae and the digestive system of the larvae are then matched. Result: The results of this study found that there was DNA transfer in larvae proved by the matching of DNA at the STR loci on the skin and the digestive system of larvae with the STR locus in blood taken from the postmortem body Conclusion: the presence of decomposed corpse DNA decomposition to the larvae of members of the order called Calliphorideae family which can be detected through the results of nuclear DNA visualization at the CSF1PO, TPOX, and TH01 loci using the PCR method.