Milligram quantities of RNA are commonly synthesized by in vitro transcription from a DNA template with T7 RNA polymerase. However, the run-off transcription method results in heterogeneity at the RNA 3′-terminus. RNA purification requires single-nucleotide resolution to separate the transcript of the correct length from the aborted or add-on transcripts that are usually present in comparable amounts. Here, we describe an RNA preparation method that uses a trans-acting DNAzyme and sequence-specific affinity column chromatography. This purification method is simple, fast, and suited for high throughput. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Cheong, H. K., Hwang, E., & Cheong, C. (2012). Rapid preparation of RNA samples using DNA-affinity chromatography and DNAzyme methods. Methods in Molecular Biology, 941, 113–121. https://doi.org/10.1007/978-1-62703-113-4_9
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