SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. Combined with the ultrahigh-throughput sequencing technologies, SuperSAGE enables analysis of millions of transcripts with lower cost and reduced effort and time. In this chapter, we present an updated protocol for this High-throughput SuperSAGE method with a special emphasis on the technique of library multiplexing.
CITATION STYLE
Matsumura, H., Yoshida, K., Luo, S., Krüger, D. H., Kahl, G., Schroth, G. P., & Terauchi, R. (2011). High-Throughput SuperSAGE. In Methods in Molecular Biology (Vol. 687, pp. 135–146). Humana Press Inc. https://doi.org/10.1007/978-1-60761-944-4_9
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