One of the major obstacles in generating induced pluripotent stem (iPS) cells suitable for therapeutic application is the low efficiency of the process and the long time required, with many iPS lines acquiring genomic aberrations. In this chapter we describe a highly efficient iPS reprogramming system based on the transient expression in pre-B cells of the transcription factor C/EBPα, followed by the induction of the four Yamanaka factors (OSKM). In addition, the process is very rapid, yielding Oct4 positive cells within 2 days and Nanog-positive iPS cell colonies within a week.
CITATION STYLE
Di Stefano, B., & Graf, T. (2016). Very rapid and efficient generation of induced pluripotent stem cells from mouse pre-B Cells. In Methods in Molecular Biology (Vol. 1357, pp. 45–56). Humana Press Inc. https://doi.org/10.1007/7651_2014_133
Mendeley helps you to discover research relevant for your work.