Expression levels of mir-30c and mir-186 in adult patients with membranous glomerulonephritis and focal segmental glomerulosclerosis

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Abstract

Background: Nephrotic syndrome is a common renal problem with different histopatho-genesis. MicroRNAs are reported to be involved in the pathophysiology of the syndrome. The aim of this study was to study the levels of miR-30c and miR-186 in NS patients. Methods: Sixty patients with primary NS (membranous glomerulonephritis (MGN, N=30) and focal segmental glomerulosclerosis (FSGS, N=30)) and 24 healthy volunteers were included. Expression levels of the miR-30c and miR-186 were evaluated in plasma and peripheral blood mononuclear cell (PBMC) samples of adult patients with NS using real-time PCR. Moreover, an in-silico analysis was performed to understand the signaling pathways and biological procedures that may be regulated by these miRNAs. Results: In the MGN group, significantly elevated levels of miR-30c and miR-186 were observed in PBMC (P= 0.037) and plasma (P= 0.035) samples, respectively. Moreover, there was a significant increase in miR-30c levels in PBMC samples of the FSGS group when compared to healthy controls (P= 0.004). In ROC curve analysis, combined levels of the studied miRNAs could discriminate cases from controls in plasma and blood cells (AUC≥0.72, P<0.05). Conclusion: A panel of miRNAs may be potential biomarkers in plasma and PBMCs samples of NS patients with different subclasses. More investigations are needed with a large sample size to validate the diagnostic values of the reported miRNAs.

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Hejazian, S. M., Ardalan, M., Shoja, M. M., Samadi, N., & Vahed, S. Z. (2020). Expression levels of mir-30c and mir-186 in adult patients with membranous glomerulonephritis and focal segmental glomerulosclerosis. International Journal of Nephrology and Renovascular Disease, 13, 193–201. https://doi.org/10.2147/IJNRD.S258624

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