MTHFR genetic polymorphism and the risk of intrauterine fetal death in Polish women

Abstract

Objectives: To evaluate the role of MTHFR genetic variants in the etiology of intrauterine fetal death in the second part of pregnancy at women from Polish population. Material and methods: A case-control study was performed on a 76 women with a positive history of at least one intrauterine fetal death after 22 gestational week and 400 healthy controls. The MTHFR genotyping for polymorphic sites 667C > T, 1298A > C, 1793G > A was determined by polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) method. Results: For 1298A > C polymorphism, no statistically significant higher frequency of AA vs. AC+CC genotype was observed in the IUFD group 67.1 % vs. 55.2% in the control group (OR = 0.61, p = 0.05, p corr = 0.15). We observed overrepresentation of three-locus haplotype CCG (p = 0.20; p corr = 0.56) and two-locus haplotype CC (p = 0.17; p corr = 0.48) in the IUFD group compared to controls. Conclusions: There was no observed relationships in genotype frequency of MTHFR 677C > T and 1793G > A variants, however 1298A > C showed a slightly higher but statistically insignificant prevalence in IUFD compared to the controls in Polish population. Further studies on a larger population are needed.