Ultrastructural localization of type V collagen in rat kidney

Abstract

Antibodies specific for the α1 (V) chain and native collagen molecules containing the α1 (V) chain have been used in electron immunohistochemical studies of rat kidney to determine the ultrastructural distribution of this class of collagen molecules. In addition, antibodies against type 1 collagen and whole basement membrane were used as markers for interstitial collagen and authentic basement membranes. Our results indicate that type V collaen is present in the renal interstitium in different forms: in close apposition to interstitial collagen fibers; in the stromal aspect of vascular basement membranes; and as particulate material not bound to other structures. On the basis of these findings, we postulate a binding or connecting function for this collagen type.