Genetic diversity of Chinese wild grape species by SSR and SRAP markers

Abstract

Simple Sequence Repeats (SSR) and Sequence-Related Amplified Polymorphism (SRAP) were used to analyze the genetic diversity of 26 genotypes from 15 Chinese wild grape species and one interspecific hybrid of Vitis L. A total of 90 alleles were amplified by ten SSR primers, and primer heterozygosity varied from 0.653 to 0.883. Twelve out of 169 SRAP primer combinations were selected for SRAP analysis. A total of 223 bands were produced, of which 208 bands were polymorphic ones; the average percentage of polymorphic bands was 93.3%. The results of clustering analysis based on SSR markers showed that most of the wild grapes could be distinguished at genetic similarity coefficient level of 0.74, and 27 genotypes could be divided into four main clusters at 0.64. The dendrogram obtained with SRAP data was almost consistent with traditional morphological classification. At the genetic similarity coefficient of 0.84, 27 genotypes could be separated. This also confirmed that V. heyneana subsp. ficifolia is a subspecies of V. heyneana, and V. adenoclada alone is a species.