Efficient transgene expression in chrysanthemum, Chrysanthemum morifolium Ramat., with the promoter of a gene for tobacco elongation factor 1 α protein

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Abstract

We investigated the usefulness of the promoter of a gene for tobacco elongation factor 1α protein (EF1 α) for transgene expression in the chrysanthemum (Chrysanthemum morifolium Ramat.). The EF1 α promoter was fused to the β-glucuronidase gene (gus) and introduced into the chrysanthemum. We obtained 238 putative transformants and GUS assay of the leaves of the in vitro plants revealed that 29.0% (69/238) of the putative plants were GUS-positive. The plants in the greenhouse that were 20 months after regeneration still showed a GUS activity in their leaves and petals. The tobacco EF1 α promoter expressed the transgene more efficiently than the 35S promoter of Cauliflower mosaic virus and could be used for transgene expression in chrysanthemum.

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Aida, R., Nagaya, S., Yoshida, K., Kishimoto, S., Shibata, M., & Ohmiya, A. (2005). Efficient transgene expression in chrysanthemum, Chrysanthemum morifolium Ramat., with the promoter of a gene for tobacco elongation factor 1 α protein. Japan Agricultural Research Quarterly, 39(4), 269–274. https://doi.org/10.6090/jarq.39.269

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