Functional genomic analysis reveals overlapping and distinct features of chronologically long-lived yeast populations

9Citations
Citations of this article
33Readers
Mendeley users who have this article in their library.

Abstract

Yeast chronological lifespan (CLS) is extended by multiple genetic and environmental manipulations, including caloric restriction (CR). Understanding the common changes in molecular pathways induced by such manipulations could potentially reveal conserved longevity mechanisms. We therefore performed gene expression profiling on several longlived yeast populations, including an ade4Δ mutant defective in de novo purine (AMP) biosynthesis, and a calorie restricted WT strain. CLS was also extended by isonicotinamide (INAM) or expired media derived from CR cultures. Comparisons between these diverse long-lived conditions revealed a common set of differentially regulated genes, several of which were potential longevity biomarkers. There was also enrichment for genes that function in CLS regulation, including a long-lived adenosine kinase mutant (ado1Δ) that links CLS regulation to the methyl cycle and AMP. Genes coregulated between the CR and ade4Δ conditions were dominated by GO terms related to metabolism of alternative carbon sources, consistent with chronological longevity requiring efficient acetate/acetic acid utilization. Alternatively, treating cells with isonicotinamide (INAM) or the expired CR media resulted in GO terms predominantly related to cell wall remodeling, consistent with improved stress resistance and protection against external insults like acetic acid. Acetic acid therefore has both beneficial and detrimental effects on CLS.

References Powered by Scopus

The hallmarks of aging

10806Citations
N/AReaders
Get full text

Genomic expression programs in the response of yeast cells to environmental changes

3942Citations
N/AReaders
Get full text

Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis

3355Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Caloric restriction extends yeast chronological life span by optimizing the Snf1 (AMPK) signaling pathway

43Citations
N/AReaders
Get full text

Emerging Omics Approaches in Aging Research

25Citations
N/AReaders
Get full text

CAN1 Arginine Permease Deficiency Extends Yeast Replicative Lifespan via Translational Activation of Stress Response Genes

17Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Wierman, M. B., Matecic, M., Valsakumar, V., Li, M., Smith, D. L., Bekiranov, S., & Smith, J. S. (2015). Functional genomic analysis reveals overlapping and distinct features of chronologically long-lived yeast populations. Aging, 7(3), 177–194. https://doi.org/10.18632/aging.100729

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 17

71%

Researcher 6

25%

Professor / Associate Prof. 1

4%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 11

48%

Biochemistry, Genetics and Molecular Bi... 9

39%

Medicine and Dentistry 2

9%

Computer Science 1

4%

Save time finding and organizing research with Mendeley

Sign up for free