Biochemical and histopathological evaluation of an in vivo model of breast cancer

Abstract

Though, the clinical management of breast cancer has improved significantly over the past 30 years, it still remains the leading cause of cancer-related female death worldwide. Prevention is the fundamental issue in breast cancer control, for which identification markers in terms of initiation and promotion are necessary. To understand this, an animal model which can recapitulate the early symptoms of breast cancer development and progression is required. Present study is an attempt to develop a convenient and economical in-vivo animal model of breast cancer suitable to conduct such study. Female Wistar and SD rats were injected with different doses and routes of administration of 7, 12-Dihydroxymethylbenz (a) anthracene (DMBA). Animals were observed for the presence of visible/palpable tumours in mammary glands. Various parameters (Tumor morphology, oxidative stress and histopathological studies were studied in different tissues (mammary, lungs, kidney, liver) after the appearance of mammary tumours in rats. After 14 weeks all the animals developed breast carcinomas. The results of this study revealed a significant difference in oxidative stress parameters between DMBA treated and control groups and these alterations were strain dependent. The H&E staining of mice mammary tissue showed development of metaplastic triple negative breast cancer. Immunohistochemistry observation confirmed the triple negative nature of mammary tumours developed in the mice. Data confirmed that DMBA can be used as breast cancer initiator and present model can be further exploited to screen potential anti-breast cancer compounds in vivo.