In vitro preparation of prefibrillar intermediates of amyloid-β and α-synuclein

Abstract

Elucidating the structural properties of early intermediates (protofibrils) on the fibril formation pathway of Aβ and a-synuclein, the structural relationship among the different intermediates and their relationship to the structure of the amyloid fibrils is critical for understanding the roles of amyloid fibril formation in the pathogenesis of Alzheimer's and Parkinson's diseases. In this chapter we discuss several methods, developed by different laboratories, that enable the preparation and stabilization of amyloid-β and a-synuclein protofibrillar species of defined morphologies for biochemical, biophysical and toxicity studies.