Cratylia mollis 1, 4 lectin: A new biotechnological tool in IL-6, IL-17A, IL-22, and IL-23 induction and generation of immunological memory

16Citations
Citations of this article
27Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Cratylia mollis lectin has already established cytokine induction in Th1 and Th2 pathways. Thereby, this study aimed to evaluate Cramoll 1, 4 in IL-6, IL-17A, IL-22, and IL-23 induction as well as analyze immunologic memory mechanism by reinducing lymphocyte stimulation. Initially we performed a screening in cultured splenocytes where Cramoll 1, 4 stimulated IL-6 production 5x more than ConA (P < 0.05). The same behavior was observed with IL-22 where the increase was greater than 4x. Nevertheless, IL-17A induction was similar for both lectins. In PBMCs, the same splenocytes course was observed for IL-6 and IL-17A. Concerning the stimulation of IL-22 and IL-23 Cramoll 1, 4 was more efficient than ConA in cytokines stimulation mainly in IL-23 (P < 0.01). Analyzing reinduced lymphocyte stimulation, IL-17A production was higher (P < 0.0 0 1) when the first stimulus was realized with Cramoll 1, 4 at 1 g/mL and the second at 5 g/mL. IL-22 shows significant differences (P < 0.01) at the same condition. Nevertheless, IL-23 revels the best response when the first stimuli was realized with Cramoll1, 4 at 100 ng/mL and the second with 5 g/mL. We conclude that the Cramoll 1, 4 is able to induce IL-6, IL-17A, IL-22, and IL-23 cytokines in vitro better than Concavalin A, besides immunologic memory generation, being a potential biotechnological tool in Th17 pathway studies. © 2013 Priscilla Stela Santana de Oliveira et al.

Cite

CITATION STYLE

APA

Oliveira, P. S. S. D., Rêgo, M. J. B. D. M., Da Silva, R. R., Cavalcanti, M. B., Galdino, S. L., Correia, M. T. D. S., … Pitta, M. G. D. R. (2013). Cratylia mollis 1, 4 lectin: A new biotechnological tool in IL-6, IL-17A, IL-22, and IL-23 induction and generation of immunological memory. BioMed Research International, 2013. https://doi.org/10.1155/2013/263968

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free