Immunotherapy has become a prominent approach for the treatment of cancer. Targeted killing of malignant cells by adoptive transfer of chimeric antigen receptor (CAR) T cells is a promising immunotherapy technique in oncology. However, the identification of cell surface antigens unique to tumor cells against which CAR T cells can be engineered has historically been challenging and not well documented in solid tumors. Here, we describe a generalized method to construct a cell subtype-specific surface antigen profile (i.e., surfaceome) from cell lines and identify high-confidence antigens as effective targets for CAR T cell therapy by integrating transcriptomics and cell surface proteomics. This method is widely applicable to all therapies utilizing CAR T cells, such as cancer, as well as infectious and autoimmune diseases.
CITATION STYLE
DeLucia, D. C., & Lee, J. K. (2020). Identification of cell surface targets for CAR T cell immunotherapy. In Methods in Molecular Biology (Vol. 2097, pp. 45–54). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0203-4_2
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