Advances in the use of lentiviral vectors for gene therapy applications have created a need for large-scale manufacture of clinical-grade viral vectors for transfer of genetic materials. Lentiviral vectors can transduce a wide range of cell types and integrate into the host genome of dividing and nondividing cells, resulting in long-term expression of the transgene both in vitro and in vivo. In this chapter, we present a method to transfect human cells, creating an easy platform to produce lentiviral vectors for CAR-T cell application.
CITATION STYLE
Moço, P. D., de Abreu Neto, M. S., Fantacini, D. M. C., & Picanço-Castro, V. (2020). Optimized Production of Lentiviral Vectors for CAR-T Cell. In Methods in Molecular Biology (Vol. 2086, pp. 69–76). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0146-4_5
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