Random mutagenesis is a useful technique to study the functions of different gene products. Propagation of the genes cloned in plasmids through a mutator strain, like Escherichia coli XL1-red, produces randomly mutagenized plasmid libraries. This method offers a very simple and economic way of introducing random point mutations throughout the gene with a fairly high mutation rate. The whole process involves transformation and propagation of a plasmid containing the desired gene into the XL-1 red strain, isolating the mutagenized plasmid library and transforming this library into a desired strain for screening the mutant phenotype. © Springer Science+Business Media, LLC 2010.
CITATION STYLE
Muteeb, G., & Sen, R. (2010). Random mutagenesis using a mutator strain. Methods in Molecular Biology, 634, 411–419. https://doi.org/10.1007/978-1-60761-652-8_29
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