Bacteriological and molecular studies on Salmonella species isolated from poultry farms

Abstract

This study comprise the examination of 100 samples of feed(40), water (36) and litter (24) collected randomly from different chicken shops, houses and farms in Kafr El-Sheikh Governorate. Results of this survey revealed isolation of 14/100(14%) Salmonella species (3)(12.5%) isolates from litter, (5)(13.8%) isolates from water and (6)(25%) isolates from farms. The prevalence rate to Salmonella was (14%) Sensitivity test was made and isolates exhibit resistance against ciprofloxacin and cefotaxime with (89%) and highly sensitivity to amikacin with (100%) so amikacin is the first drug choice for treatment of Salmonella infection. A PCR based assay was developed to developed to detect the prevalence of Salmonella in samples and to evaluate plasmid and chromosome-borne virulence genes (stn-invA) which considered as a target genes for the detection of Salmonella The results of detection of resistance genes (qnr S-blaTEM) as a resistance genes in all isolates of Salmonella.