Effect of nitrogen addition on the carbon metabolism of soil microorganisms in a Calamagrostis angustifolia wetland of the Sanjiang Plain, northeastern China

Abstract

Purpose: Soil microorganisms are important mediators of land ecosystem functions and stability. However, carbon sources in different amounts of nitrogen addition are known to affect the function of soil microbial communities. Thus, this study sought to evaluate the effects of nitrogen addition on the carbon utilization capacity of soil microorganisms in the Sanjiang Plain wetland, northeastern China. Methods: Three nitrogen treatments (CK, 0 kg N ha−1 a−1; N40, 40 kg N ha−1 a−1; and N80 kg N ha−1 a−1) were evaluated in the Honghe National Nature Reserve of the Sanjiang Plain. The carbon metabolism capacity of soil microorganisms in the C. angustifolia wetland was investigated after five consecutive year’s nitrogen addition treatment using the Bio-Eco technique. Results: Different amounts of nitrogen addition conditions resulted in significant differences in pH, ammonium nitrogen (NH4+), dissolved organic carbon (DOC), and soil microbial alpha diversity. The average well-color development (AWCD) in the Bio-Eco Plate assay increased gradually with incubation time, and different nitrogen levels significantly affected these AWCD values (P < 0.05), with the N40 treatment exhibiting the highest value. Furthermore, the N80 treatment had significantly lower Shannon and Pielou diversity indices (P < 0.05). N40 significantly promoted carbohydrate, amino acid, and ester utilization rates by soil microorganisms, whereas N80 significantly inhibited carbohydrate, amino acid, alcohol, amine, and organic acids utilization. Redundancy analysis (RDA) showed that the three treatments had remarkable differences in soil microbial community metabolism, and the cumulative variance contribution was 72.86%. In addition, RDA revealed that the N80 treatment was positively correlated with the TN, SMC, DON, and TOC but negatively correlated with DOC, NH4+, pH, and NO3−. Conclusion: Long-term nitrogen addition leads to changes in soil microbial community structure and significantly alters the ability of soil microorganisms to utilize carbon sources in the Calamagrostis angustifolia wetland.