Aderência bacteriana in vitro a lentes intra-oculares de polimetilmetacrilato e de silicone

Abstract

Purpose: To evaluate bacterial adherence to silicone and polymetylmethacrylate (PMMA) intraocular lenses as a risk factor for postsurgery endophthalmitis by using an in vitro model with three potentially pathogenic microorganisms. Methods: In vitro experiments were carried out with the Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis (clinical isolate) and Pseudomonas aeruginosa ATCC 27853 strains including growth curves, tests to verify capsule production, hydrophobicity and adherence to different materials as well as optical microscopy, scanning electronic microscopy (SEM) and atomic force microscopy (AFM). Results: No relation between capsule production, adherence of the strains tested and amount of microorganisms was observed; no statistically significant differences were detected between S. aureus and S. epidermidis adherence to polymetylmethacrylate and silicone intraocular lenses; P. aeruginosa was the most adherent microorganism to both materials. This adherence pattern was confirmed by SEM, while biofilm production by the three strains was visualized by AFM. Conclusions: In vitro experiments showed no differences of bacterial adherence between PMMA and silicone lenses, but P. aeruginosa displayed a greater level of adherence in relation to staphylococci. All three strains were shown to produce biofilm. Silicone was shown to be more hydrophobic when compared to polymethylmethacrylate.