Quantitative Fluorescence Confocal Laser Scanning Microscopy (CLSM)

  • Sandison D
  • Williams R
  • Wells K
  • et al.
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Abstract

The confocal imaging geometry provides a dramatic optical advantage for fluorescence microscopy by discriminating against out-of-focus background with minimal loss of image-forming signal. Significant enhancement of both axial and lateral imaging resolution is also...

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Sandison, D. R., Williams, R. M., Wells, K. S., Strickler, J., & Webb, W. W. (1995). Quantitative Fluorescence Confocal Laser Scanning Microscopy (CLSM). In Handbook of Biological Confocal Microscopy (pp. 39–53). Springer US. https://doi.org/10.1007/978-1-4757-5348-6_3

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