Antimicrobial activity of senna alata (L.), rhinacanthus nasutus and chromolaena odorata (L.) collected in southern vietnam

Abstract

This study aimed to evaluate the antimicrobial activity of Senna alata (L.), Rhinacanthus nasutus and Chromolaena odorata against antibiotic resistant Staphylococcus aureus, Pseudomonas aeruginosa clinical strains; vitro – induced antibiotic resistant S. aureus strains; S. aureus ATCC 25213 and P. aeruginosa ATCC 9027. Plant samples were extracted with different solvents including ethanol, ethyl acetate and n-hexane. Disc diffusion method was used to evaluate the antimicrobial activity of extracts. In general, most of extracts showed good antimicrobial activity against both antibiotic resistant and sensitive S. aureus but no activity against P. aeruginosa. To clinical S. aureus strains, C. odorata and R. nasutus extracts using ethyl acetate showed better inhibitory zones (17.17 ± 0.29 mm; 16.67 ± 0.58 mm) than the ones extracted using ethanol (14.17 ± 0.58mm, 11.67 ± 1.53 mm) and n- hexane (12.17 ± 0.58 mm; 13.67 ± 0.58 mm, respectively). To other S. aureus strains, S. alata (L.) extract using ethanol showed the greatest inhibition zone (22.33 ± 0.58 mm). This result suggested that these traditional herbs should be further investigated to serve as alternative treatment for multidrug- resistant S. aureus infections and probably also for other gram positive pathogenic bacteria.