Cap analysis of gene expression (CAGE) provides accurate high-throughput measurement of RNA expression. By the large-scale analysis of 5′ end of transcripts using CAGE method, it enables not only determination of the transcription start site but also prediction of promoter region. Here we provide a protocol for the construction of no-amplifi cation non-tagging CAGE libraries for Illumina next-generation sequencers (nAnT-iCAGE). We have excluded the commonly used PCR amplifi cation and cleavage of restriction enzyme to eliminate any potential biases. As a result, we achieved less biased simple preparation process.
CITATION STYLE
Murata, M., Nishiyori-Sueki, H., Kojima-Ishiyama, M., Carninci, P., Hayashizaki, Y., & Itoh, M. (2014). Detecting expressed genes using CAGE. Methods in Molecular Biology, 1164, 67–85. https://doi.org/10.1007/978-1-4939-0805-9_7
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