Production of uronic acids by hydrothermolysis of pectin as a model substance for plant biomass waste

Abstract

The hydrolysis of high methyl ester citrus-apple pectin as a model substance for plant biomass waste rich in pectin fraction resulting in an uronic acids was performed in a batch reactor using subcritical water. The effects of the reaction temperature and time on the composition of the products contained in the separated liquid fractions were studied. The optimal experimental design methodology was used for modelling and optimizing the yield of uronic acids. In good agreement with experimental results (R2 = 0.986), the model predicts an optimal yield of uronic acids (approx. 77.3 g kg-1 ± 0.7 g kg-1) at a temperature and a time of about 155°C and 36 min. Uronic acids were isolated from reaction mixture using the ion exchange method. Higher temperature and longer holding time resulted in a greater degree of thermal degradation of uronic acids and simultaneously higher yield of losses and gas fractions