Significant effort has been devoted to the optimization of solid-phase peptide synthesis (SPPS) to maximize the process to facilitate the synthesis of a desired peptide sequence, without extensive optimization or resynthesis. Over the last 25 years, a set of synthetic protocols developed by Kent and Alewood has proven to be robust and efficient for Boc/Bzl SPPS and has been widely adopted by the research community. In this chapter, we describe a variation of manual in situ neutralization protocols for Boc-SPPS that are highly effective for the rapid synthesis of peptides with different C-terminal functionalities.
CITATION STYLE
Adhikary, R., & Dawson, P. E. (2020). In situ neutralization protocols for Boc-SPPS. In Methods in Molecular Biology (Vol. 2103, pp. 29–40). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0227-0_3
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