New strategies for evaluation and analysis of SELEX experiments

21Citations
Citations of this article
73Readers
Mendeley users who have this article in their library.

Abstract

Aptamers are an interesting alternative to antibodies in pharmaceutics and biosensorics, because they are able to bind to a multitude of possible target molecules with high affinity. Therefore the process of finding such aptamers, which is commonly a SELEX screening process, becomes crucial. The standard SELEX procedure schedules the validation of certain found aptamers via binding experiments, which is not leading to any detailed specification of the aptamer enrichment during the screening. For the purpose of advanced analysis of the accrued enrichment within the SELEX library we used sequence information gathered by next generation sequencing techniques in addition to the standard SELEX procedure. As sequence motifs are one possibility of enrichment description, the need of finding those recurring sequence motifs corresponding to substructures within the aptamers, which are characteristically fitted to specific binding sites of the target, arises. In this paper a motif search algorithm is presented, which helps to describe the aptamers enrichment in more detail. The extensive characterization of target and binding aptamers may later reveal a functional connection between these molecules, which can be modeled and used to optimize future SELEX runs in case of the generation of target-specific starting libraries. © 2014 Rico Beier et al.

References Powered by Scopus

DNA sequencing with chain-terminating inhibitors.

59262Citations
N/AReaders
Get full text

Sequencing technologies the next generation

5476Citations
N/AReaders
Get full text

ViennaRNA Package 2.0

3425Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Three decades of nucleic acid aptamer technologies: Lessons learned, progress and opportunities on aptamer development

396Citations
N/AReaders
Get full text

Aptamers in analytics

199Citations
N/AReaders
Get full text

New technologies provide quantum changes in the scale, speed, and success of SELEX methods and aptamer characterization

145Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Beier, R., Boschke, E., & Labudde, D. (2014). New strategies for evaluation and analysis of SELEX experiments. BioMed Research International, 2014. https://doi.org/10.1155/2014/849743

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 32

59%

Researcher 20

37%

Professor / Associate Prof. 1

2%

Lecturer / Post doc 1

2%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 21

41%

Biochemistry, Genetics and Molecular Bi... 21

41%

Chemistry 6

12%

Engineering 3

6%

Save time finding and organizing research with Mendeley

Sign up for free