Testing of large populations for virus infection is now a reality worldwide due to the coronavirus (SARS-CoV-2) pandemic. The demand for SARS-CoV-2 testing using alternatives other than PCR led to the development of mass spectrometry (MS)-based assays. However, MS for SARS-CoV-2 large-scale testing have some downsides, including complex sample preparation and slow data analysis. Here, we describe a high-throughput targeted proteomics method to detect SARS-CoV-2 directly from nasopharyngeal and oropharyngeal swabs. This strategy employs fully automated sample preparation mediated by magnetic particles, followed by detection of SARS-CoV-2 nucleoprotein peptides by turbulent flow chromatography coupled with tandem mass spectrometry.
CITATION STYLE
Viana, L. G., Lebkuchen, A., Schuch, R. A., Okai, G. G., Salgueiro, J. S., Cardozo, K. H. M., & Carvalho, V. M. (2022). Mass Spectrometry Multiplexed Detection of SARS-CoV-2. In Methods in Molecular Biology (Vol. 2511, pp. 161–174). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2395-4_12
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