Biochemical analysis of metallo-β-lactamase NDM-3 from a multidrug-resistant escherichia coli strain isolated in japan

Abstract

New Delhi metallo-β-lactamase-3 (NDM-3) was identified in a multidrug-resistant Escherichia coli isolate, NCGM77, obtained from the feces of a patient in Japan. The enzymatic activities of NDM-3 against β-lactams were similar to those of NDM-1, although NDM-3 showed slightly lower k cat/Km ratios for all the β-lactams tested except for doripenem. The genetic context for blaNDM-3 was tnpA-bla NDM-3-bleMBL-trpF-dsbC-tnpA-sulI-qacEdeltaI-AadA2-dfrA1, which was present on an approximately 250-kb plasmid. © 2014, American Society for Microbiology.