Antimicrobial Activity of a Novel Silane Coupling Agent Consisting of a Quaternary Ammonium Salt Using a Polymicrobial Biofilm Model

Abstract

The purpose of this investigation was to evaluate the antimicrobial activity of a novel quaternary ammonium silane coupling agent, N-allyl-N-decyl-N-methyl-N-trimethoxysilylpropylammonium iodide (10-I), against early stage biofilms using a polymicrobial biofilm model that simulates oral plaque-like biofilm formation on a solid phase. Cover glasses were immersed in a sufficient volume of the 800-ppm solution of 10-I for 1 h to modify the surface. Aliquots of a mixture of stimulated saliva from a single subject and McBain medium were dispensed into polystyrene 24-well cell culture plates and then cultured at 37.0°C under anaerobic condition. After culturing, we calculated colony forming unit (CFU). CFU of the 10-I group was approximately 80% lower than that of the control group, demonstrating strong antimicrobial activity. Therefore, surface modification with 10-I is effective for treating oral indigenous bacteria-related dental diseases found in the elderly and immunocompromised individuals and possible systemic complications, such as aspiration pneumonia.