Plant tissues contain high levels of nonprotein contaminants such as lipids, phenolic compounds, and polysaccharides among others, which interfere with protein extraction and electrophoretic separation. Preparation of good-quality protein extracts is a critical issue for successful electrophoretic analysis. Here, we describe a three-step method for protein extraction from lipid-rich plant tissues, which is suitable for both 1-D and 2-D electrophoresis and is compatible with downstream applications. The protocol includes prefractionation, filtration, and TCA/acetone precipitation steps prior to protein resolubilization. © 2014 Springer Science+Business Media, LLC.
CITATION STYLE
Zienkiewicz, A., Rejón, J. D., De Dios Alché, J., Rodríguez-García, M. I., & Castro, A. J. (2014). A protocol for protein extraction from lipid-rich plant tissues suitable for electrophoresis. Methods in Molecular Biology, 1072, 85–91. https://doi.org/10.1007/978-1-62703-631-3_7
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