A novel version of bead -based assays with fluorescence detection enables the high-throughput analysis of antibodies and proteins. The protocols are carried out in special 384-well plates, require very few manual interventions, and are easy to automate. Here we describe how the technology can be used to determine antibody titers and screen for product glycosylation, a critical quality attribute, early in cell line and bioprocess development.
CITATION STYLE
Giehring, S. (2020). High-throughput quantification and glycosylation analysis of antibodies using bead-based assays. In Methods in Molecular Biology (Vol. 2095, pp. 271–284). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0191-4_15
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