Antibacterial and Antibiofilm Activity of Calcite (CaCO3) Nanoparticles Synthesized from Chicken Eggshell

Abstract

Background: The Calcite Calicium Carbonate Nanoparticles (CaCO3NPs) synthesized from the chicken eggshell (CES), this was done by crushing the shell and sieving. Objective: The aim of the current study is to find a way to benefit from CES residues and the possibility of exploiting them in the production of CaCO3NPs, which is used in many medical applications. Material and methods: A hundred burn wounds samples were collected from several clinical sources. A total of 60 bacterial isolates were obtained from the burn wounds. CaCO3NPs has effective antibacterial activity; the inhibition zone was 12 mm for Acinetobacter baumannii, Proteus mirabilis and Staphylococcus aureus, 13 mm for Escherichia coli, 11 mm for Klebsiella pneumoniae, 14 mm for Burkholderia cepacia, and Pseudomonas aeruginosa. Results: The results showed that the MIC for A. baumannii was 25 µg/ml. The same MIC values were for K. pneumoniae and S. aureus, while B. cepacia, E. coli, P. mirabilis, and P. aeruginosa have the MIC was 6.25 µg/ml. The present study shows the efficiency of CaCO3NPs to prevent the biofilms of Gram negative and Gram-positive isolates. Conclusion: Synthesized CaCO3NPs from the chicken eggshell have biological effectiveness and it can be used in dental fillings and bone repair.