Molecular characterization and toxin-typing of Clostridium difficile isolates of dogs and pigs from Assam and Mizoram of North East India

Abstract

Clostridium difficile with its virulence factors A and B toxins cause Pseudomembranous colitis. Bacterium was isolated from 57 dog and 41 pig diarrheic faecal samples in cycloserinecefaxitin fructose agar media and molecular detection was done by amplification of gluD gene (755 bp). Variability of toxin genes in positive isolates was tested by multiplex PCR. Detection of binary toxin genes (cdtA and cdtB) was also done. Results showed 33.67% positivity with 18 and 15 from dog and pig respectively, from which 10 and 5 were toxigenic and 11 pig isolates exhibited binary toxin. PCR-RFLP demonstrated toxinotype 0 in all A+B+ isolates.