Green synthesis of copper oxide nanoparticles using Bryophyllum pinnatum leaf extract and its antibacterial potential against Listeria monocytogenes

Abstract

Green synthesis nanoparticle preparation is a biocompatible, safe, and ecologically friendly method of nanoparticle preparation. In the green manufacture of nanoparticles, plants and other microorganisms such as bacteria, algae, and fungi are employed. In terms of efficiency, speed, affordability, non-toxicity, simplicity, and environmental friendliness, it outperforms physicochemical techniques. Nanoparticle production mediated by plant biomass is a fast expanding topic of research around the world. Copper oxide nanoparticles were made from the aqueous leaf extract of the Bryophyllum pinnatum plant in this work. The antibacterial activity of the produced copper oxide nanoparticles was investigated against Listeria monocytogenes. To make copper oxide nanoparticles, 1ml aqueous extract of Bryophyllum pinnatum was mixed with 20ml (0.1M) Cu(NO3)2 and centrifuged at 10,000 rpm for 15 minutes before being rinsed with distilled water and dried. UV-Visible spectroscopy, High-resolution field emission scanning electron microscope (HR FESEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Dynamic light scattering (DLS) techniques were used to evaluate the produced copper oxide nanoparticles. The antibacterial potential of the compound was next tested utilizing disk diffusion and agar well diffusion methods against Listeria monocytogenes.