Mathematical models are important tools in helping us to understand complex biological systems. Models of phytochrome-regulated systems in Arabidopsis thaliana have shown the importance of dimerization, nuclear transport, and thermal/dark reversion in mediating phytochrome activity and plant development. Here we go through the steps required to calculate the steady-state amounts of phytochrome subspecies relative to the total phytochrome molecule population. Starting from a simplified two-state system we expand and apply the technique to the extended phytochrome dimer model. Additionally, we provide a Python package that can automatically calculate the proportion of phytochrome B in a particular state given specific experimental conditions.
CITATION STYLE
Smith, R. W., & Fleck, C. (2019). Basic Phytochrome B Calculations. In Methods in Molecular Biology (Vol. 2026, pp. 121–133). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9612-4_9
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