We describe a method to detect influenza virus using an evanescent-field-activated fluorescence scanner type glycan array and ELISA system. Neoglycoprotein was prepared by combination of organic chemistry and biomaterial preparation. These ligands were spotted on a glass plate or plastic well to make a glycan array and ELISA plate. We detected cultured influenza virus using glycan array and ELISA. Then, we showed that the neoglycoprotein binds to Cy3-labeled hemagglutinins (H1 and H5), a NeuAcα2,6LacNAc or NeuAcα2,3LacNAc recognized protein, as detected.
CITATION STYLE
Nakakita, S. ichi, Sriwilaijaroen, N., Suzuki, Y., & Hirabayashi, J. (2020). Preparation and detection of glycan-binding activity of influenza virus. In Methods in Molecular Biology (Vol. 2132, pp. 567–583). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0430-4_49
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