In order to facilitate strain improvement by concerted breeding in the cephalosporin producing imperfect filamentous fungus Cephalo sporium acremonium, it is attempted to develop a eukaryotic vector for molecular cloning based on mitochondrial (mt) DNA. Fragments of mtDNA from C. acremonium were inserted into a yeast/bacterial hybrid plasmid (pDAM1) lacking a eukaryotic replicon. Six hybrid plasmids were obtained each containing a different mt fragment which together comprise about 60% of the total mtDNA. One of these recombinant plasmid vectors (pCP2) showed a high replication efficiency which is comparable to that of vectors containing yeast 2 μm DNA. This plasmid therefore fulfills the requirements for practical application. © 1982 Springer-Verlag.
CITATION STYLE
Tudzynski, P., & Esser, K. (1982). Extrachromosomal genetics of Cephalosporium acremonium - II. Development of a mitochondrial DNA hybrid vector replicating in Saccharomyces cerevisiae. Current Genetics, 6(2), 153–158. https://doi.org/10.1007/BF00435215
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