Detection of Escherichia coli O157: H7 and Salmonella in ground beef by a bead-free quantum dot-facilitated isolation method

34Citations
Citations of this article
53Readers
Mendeley users who have this article in their library.
Get full text

Abstract

The aims of this study were to introduce a new immunological bead-free cell detection method using quantum dots (QDs) as reporter markers for foodborne pathogen detection. QDs are nanosized particles with long-term photostability, high quantum yield, broad absorption spectra, and narrow, symmetric emission and high signal-to-noise ratio. The chemical compound [(1-ethyl-3-3-dimethylaminopropyl) carbodiimide hydrochloride] (EDC) and protein A were used as crosslinkers for manufacturing QD-antibody conjugates. To minimize the inhibition of QD fluorescence by the magnetic beads, the beads were removed after the primary pathogen isolation and before fluorescence measurement. Detection signals were increased four-fold after employing the bead-free isolation method. With a 24-h enrichment, the bead-free QD-facilitated detection method was able to detect 10. CFU/g Escherichia coli O157:H7 and Salmonella from artificially contaminated ground beef. To our knowledge, this detection method is the first research that combined a new EDC-protein A QD-labeling technique and bead-free fluorescence measurement to detect E. coli O157:H7 and Salmonella in ground beef. © 2012 Elsevier B.V.

Cite

CITATION STYLE

APA

Wang, L., Wu, C. S., Fan, X., & Mustapha, A. (2012). Detection of Escherichia coli O157: H7 and Salmonella in ground beef by a bead-free quantum dot-facilitated isolation method. International Journal of Food Microbiology, 156(1), 83–87. https://doi.org/10.1016/j.ijfoodmicro.2012.03.003

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free