Melanopsin-expressing, intrinsically photosensitive retinal ganglion cells (ipRGCs) are a relatively recently discovered class of photoreceptor. ipRGCs can be subdivided into at least five subtypes (M1-M5), each of which has a distinct complement of morphological and physiological properties. ipRGC subtypes can be identified morphologically based on a combination of dendritic morphology and immunostaining for a cell-type specific marker. In this chapter, we describe methods for conclusively identifying each of the five ipRGC subtypes through a combination of patch clamp electrophysiology, Neurobiotin filling, visualization of ipRGC dendrites, and immunostaining for the marker SMI-32.
CITATION STYLE
Lee, S. K., & Schmidt, T. M. (2018). Morphological identification of melanopsin-expressing retinal ganglion cell subtypes in mice. In Methods in Molecular Biology (Vol. 1753, pp. 275–287). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7720-8_19
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