Seroprevalence of anti-Toxocara canis antibodies and associated risk factors among dog owners in the rural community of Nakhon Si Thammarat province, southern Thailand

Abstract

Background: Human toxocariasis is a zoonotic parasitic disease caused by the Toxocara canis and T. cati nematodes larvae. Dog owners are at a higher risk of acquiring T. canis infection, and there is no available evidence regarding the seroprevalence of T. canis infection among dog owners in Thailand. Therefore, this study aimed to investigate the seroprevalence of T. canis infection and associated risk factors among dog owners in rural areas of Thailand. Methods: A total of 132 dog owners, including 25 men and 107 women, were recruited for this study. Serum anti-T. canis IgG antibodies were detected using a commercial enzyme-linked immunosorbent assay (ELISA) kit, and information on risk factors was collected using a questionnaire. In addition, hematological parameters were analyzed by the auto hematology analyzer. Risk variables associated with T. canis infection were investigated using univariate and multivariate logistic regression models. Results: The overall seroprevalence of T. canis was 76.5% (101/132). Men were more likely to be infected with T. canis than women. Univariate analysis revealed that dog owners who did not practice handwashing before meals (p = 0.005) or after contact with soil (p = 0.035) or dogs (p = 0.049) had a substantially higher risk of acquiring T. canis infection. After adjusting for confounders, not practicing handwashing before meals remained a significant risk factor for T. canis infection (p = 0.038). The mean number of eosinophils was significantly higher in the seropositive group than in the seronegative group. Conclusions: This is the first serological report of T. canis infection among dog owners reflecting the high rate of T. canis seropositivity in rural areas of southern Thailand. This study also provides group-specific data concerning modifiable risk behaviors for more effective T. canis infection control and prevention strategies in Thailand.