Super-resolution microscopy (SRM) can provide a window on the nanoscale events of virus replication. Here we describe a protocol for imaging hepatitis C virus-infected cells using localization SRM. We provide details on sample preparation, immunostaining, data collection, and super-resolution image reconstruction. We have made all efforts to generalize the protocol to make it accessible to all budding super-resolution microscopists.
CITATION STYLE
Pereira, P. M., Jacobs, C., & Grove, J. (2019). Investigating hepatitis C virus infection using super-resolution microscopy. In Methods in Molecular Biology (Vol. 1911, pp. 247–261). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8976-8_17
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