Promoter architecture and promoter engineering in saccharomyces cerevisiae

74Citations
Citations of this article
242Readers
Mendeley users who have this article in their library.

Abstract

Promoters play an essential role in the regulation of gene expression for fine-tuning genetic circuits and metabolic pathways in Saccharomyces cerevisiae (S. cerevisiae). However, native promoters in S. cerevisiae have several limitations which hinder their applications in metabolic engineering. These limitations include an inadequate number of well-characterized promoters, poor dynamic range, and insufficient orthogonality to endogenous regulations. Therefore, it is necessary to perform promoter engineering to create synthetic promoters with better properties. Here, we review recent advances related to promoter architecture, promoter engineering and synthetic promoter applications in S. cerevisiae. We also provide a perspective of future directions in this field with an emphasis on the recent advances of machine learning based promoter designs.

Cite

CITATION STYLE

APA

Tang, H., Wu, Y., Deng, J., Chen, N., Zheng, Z., Wei, Y., … Keasling, J. D. (2020, August 1). Promoter architecture and promoter engineering in saccharomyces cerevisiae. Metabolites. MDPI AG. https://doi.org/10.3390/metabo10080320

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free